Immunohistochemical analysis of CD45RO+ T cells and vascular endothelial growth factor expression in cyclosporin A-induced rat gingival tissue
| Autor: | Mustafa Cihat Avunduk, Kemal Üstün, Tamer Ataoglu, Nilgun Ozlem Alptekin, Emre Yaprak |
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| Rok vydání: | 2011 |
| Předmět: |
Molar
CD4-Positive T-Lymphocytes Male Vascular Endothelial Growth Factor A medicine.medical_specialty CD4 antigen CD4-CD8 Ratio Biology CD8-Positive T-Lymphocytes Rats Sprague-Dawley chemistry.chemical_compound T-Lymphocyte Subsets Cyclosporin a Internal medicine medicine Animals Gingival tissue Gingival Overgrowth Rats Vascular endothelial growth factor Vascular endothelial growth factor A Endocrinology chemistry Immunology Cyclosporine Periodontics Immunohistochemistry Leukocyte Common Antigens Immunologic Memory CD8 |
| Zdroj: | Journal of periodontology. 83(2) |
| ISSN: | 1943-3670 |
| Popis: | The aim of this study is to evaluate CD4(+), CD8(+), and CD45RO(+) T cells, and vascular endothelial growth factor (VEGF) expression in cyclosporin A (CsA)-induced rat overgrown gingival tissue during an 8-week period.Sixty male Sprague-Dawley rats weighing 200 to 250 g were used in this study. Mandibular first molars were ligated with 3-0 silk suture. The rats received daily doses of 0.09% NaCl (control group) or 10 mg/kg body weight of CsA (test group) by intraperitoneal injections. Five rats from the control group and 10 rats from the test group were sacrificed at each experimental period (2, 4, 6, and 8 weeks after the beginning of CsA treatment). The specimens were examined immunohistochemically.CD4(+), CD8(+), and CD45RO(+) T cells, and VEGF expression were more prevalent in the CsA-treated group than in the control group (P0.05). VEGF was significantly correlated with CD4(+) T cells, CD4(+)/CD8(+) ratio, and CD45RO(+) cells (P0.05).Based on our findings, we conclude that VEGF, a major regulator of angiogenesis, and CD4(+), CD8(+), and CD45RO(+) memory T cells play a key role in CsA-induced gingival overgrowth. |
| Databáze: | OpenAIRE |
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