Acriflavine-loaded solid lipid nanoparticles: preparation, physicochemical characterization, and anti-proliferative properties
| Autor: | Somayeh Vandghanooni, Sattar Akbari Nakhjavani, Forough Rasoulian, Morteza Eskandani, Masoud Eskandani |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
Cell Survival Chemistry Pharmaceutical Pharmaceutical Science medicine.disease_cause digestive system chemistry.chemical_compound Antiseptic Solid lipid nanoparticle medicine Humans Acriflavine Particle Size Cytotoxicity Cell Proliferation Chemistry RNA General Medicine Anti proliferative Fluorescence digestive system diseases Biochemistry A549 Cells Liposomes Anti-Infective Agents Local Nanoparticles Genotoxicity |
| Zdroj: | Pharmaceutical development and technology. 26(9) |
| ISSN: | 1097-9867 |
| Popis: | Acriflavine (ACF) is an antiseptic compound with the potential antitumor activity which is used for the fluorescent staining of RNA due to its dominant fluorescent emission at ∼515 nm. Here, solid lipid nanoparticles (SLNs) containing ACF (ACF-SLNs) were prepared and their physicochemical properties, potential geno/cytotoxicity, as well as the fluorescent properties were investigated. FITC-annexin V/PI staining and cell cycle assays were carried out to find the type of cellular death caused. Particle size analysis and SEM images revealed that spherical ACF-SLNs had a homogeneous dispersion with a mean diameter of 106 ± 5.7 nm. Drug loading (DL) of 31.25 ± 4.21 mg/mL and high encapsulation efficiency (EE%) (89.75 ± 5.44) were found. ACF-SLNs physically were relatively stable in terms of dispersion, size, and EE. The uptake study demonstrated the potential use of fluorescent ACF-SLNs in bio-distribution studies. MTT assay showed that plain ACF could induce growth inhibition of A549 cells with |
| Databáze: | OpenAIRE |
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