High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis
| Autor: | Carrere-Kremer, Severine, Rubbo, Pierre-Alain, Pisoni, Amandine, Bendriss, Sophie, Marin, Grégory, Peries, Marianne, Bolloré, Karine, Terru, Dominique, Godreuil, Sylvain, Bourdin, Arnaud, Van De Perre, Philippe, Tuaillon, Edouard, Herrmann, Jean Louis |
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| Přispěvatelé: | Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Université de Montpellier (UM), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
Male
Bacterial Diseases 0301 basic medicine Physiology Polymers medicine.medical_treatment lcsh:Medicine [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity White Blood Cells 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Animal Cells Immune Physiology Odds Ratio Medicine and Health Sciences Interferon gamma 030212 general & internal medicine lcsh:Science Immune Response Innate Immune System Multidisciplinary biology Latent tuberculosis T Cells Middle Aged 3. Good health Actinobacteria Chemistry Infectious Diseases Cytokine Macromolecules Physical Sciences Cytokines Female Cellular Types Interferon-gamma Release Tests Research Article medicine.drug Adult Materials by Structure Immune Cells Immunology Materials Science 030106 microbiology Peripheral blood mononuclear cell Microbeads Mycobacterium tuberculosis Interferon-gamma 03 medical and health sciences Bacterial Proteins Latent Tuberculosis Diagnostic Medicine medicine Humans Tuberculosis Secretion Interleukin 4 Aged Antigens Bacterial Blood Cells Bacteria lcsh:R Organisms Biology and Life Sciences Cell Biology Molecular Development Tropical Diseases Polymer Chemistry bacterial infections and mycoses biology.organism_classification medicine.disease Immune System Leukocytes Mononuclear lcsh:Q Cytokine secretion Reagent Kits Diagnostic Physiological Processes Mycobacterium Tuberculosis Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (9), pp.e0162137. ⟨10.1371/journal.pone.0162137⟩ PLoS ONE, Vol 11, Iss 9, p e0162137 (2016) |
| ISSN: | 1932-6203 |
| Popis: | International audience; Interferon gamma (IFN-γ) release assays (IGRAs) detect Mycobacterium tuberculosis (Mtb) infection regardless of the active (ATB) or latent (LTBI) forms of tuberculosis (TB). In this study, Mtb-specific T cell response against region of deletion 1 (RD1) antigens were explored by a microbead multiplex assay performed in T-SPOT TB assay (T-SPOT) supernatants from 35 patients with ATB and 115 patients with LTBI. T-SPOT is positive when over 7 IFN-γ secreting cells (SC)/250 000 peripheral blood mononuclear cells (PBMC) are enumerated. However, over 100 IFN-γ SC /250 000 PBMC were more frequently observed in the ATB group compared to the LTBI group. By contrast, lower cytokine concentrations and lower cytokine productions relative to IFN-γ secretion were observed for IL 4, IL-12, TNF-α, GM-CSF, Eotaxin and IFN-α when compared to LTBI. Thus, high IFN-γ release and low cytokine secretions in relation with IFN-γ production appeared as signatures of ATB, corroborating that multicytokine Mtb-specific response against RD1 antigens reflects host capacity to contain TB reactivation. In this way, testing cytokine profile in IGRA supernatants would be helpful to improve ATB screening strategy including immunologic tests. |
| Databáze: | OpenAIRE |
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