Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)
Autor: | Hyun-Seok Moon, Sang-Heon Kim, Moon-Young Yoon, Jae-Ho Ko, A-Ru Kim, Yeongmi Choi |
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Rok vydání: | 2020 |
Předmět: |
lcsh:Biotechnology
Aptamer Clinical Biochemistry Biomedical Engineering Oxide DNA Single-Stranded Serum Albumin Human 030209 endocrinology & metabolism Sequence (biology) Biosensing Techniques 02 engineering and technology reduced graphene oxide Article Fluorescence Analytical Chemistry law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law lcsh:TP248.13-248.65 glycated human serum albumin medicine Humans Instrumentation Engineering (miscellaneous) Detection limit diabetes SELEX Graphene SELEX Aptamer Technique aptamer General Medicine 021001 nanoscience & nanotechnology Human serum albumin chemistry Biophysics Graphite 0210 nano-technology Systematic evolution of ligands by exponential enrichment Biotechnology medicine.drug |
Zdroj: | Biosensors, Vol 10, Iss 141, p 141 (2020) Biosensors Volume 10 Issue 10 |
ISSN: | 2079-6374 |
Popis: | Diabetes is one of the top 10 global causes of death. About one in 11 global adults have diabetes. As the disease progresses, the mortality rate increases, and complications can develop. Thus, early detection and effective management of diabetes are especially important. Herein, we present a novel glycated human serum albumin (GHSA) aptamer, i.e., GABAS-01, which has high affinity and specificity. The aptamer was selected by reduced graphene oxide-based systematic evolution of ligands by exponential enrichement (rGO-based SELEX) against GHSA. After five rounds of selection through gradually harsher conditions, GABAS-01 with high affinity and specificity for the target was obtained. GABAS-01 was labeled by FAM at the 5&prime end and characterized by measuring the recovery of a fluorescence signal that is the result of fluorescence quenching effect of rGO. As a result, GABAS-01 had low-nanomolar Kd values of 1.748 ± 0.227 nM and showed a low limit of detection of 16.40 &mu g/mL against GHSA. This result shows the potential application of GABAS-01 as an effective on-site detection probe of GHSA. In addition, these properties of GABAS-01 are expected to contribute to detection of GHSA in diagnostic fields. |
Databáze: | OpenAIRE |
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