Effects of Wild Yam Root (Dioscorea villosa) Extract on the Gene Expression Profile of Triple-negative Breast Cancer Cells
| Autor: | Abdulaziz Almalki, Karam F.A. Soliman, Elizabeth Mazzio, Selina Darling-Reed |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
biology Dioscorea Plant Extracts Kinase Gene Expression Triple Negative Breast Neoplasms Cell cycle Steroid biosynthesis biology.organism_classification Biochemistry Molecular biology Transcriptome Cyclin-dependent kinase Dioscorea villosa Gene expression Genetics biology.protein Humans Molecular Biology Research Article Cyclin |
| Zdroj: | Cancer Genomics Proteomics |
| ISSN: | 1790-6245 1109-6535 |
| Popis: | Background/aim Wild yam extract [Dioscorea villosa, (WYE)] is consistently lethal at low IC50s across diverse cancer-lines in vitro. Unlike traditional anti-cancer botanicals, WYE contains detergent saponins which reduce oil-water interfacial tensions causing disintegration of lipid membranes and causing cell lysis, creating an interfering variable. Here, we evaluate WYE at sub-lethal concentrations in MDA-MB-231 triple-negative breast cancer (TNBC) cells. Materials and methods Quantification of saponins, membrane potential, lytic death and sub-lethal WYE changes in whole transcriptomic (WT) mRNA, miRNAs and biological parameters were evaluated. Results WYE caused 346 differentially expressed genes (DEGs) out of 48,226 transcripts tested; where up-regulated DEGS reflect immune stimulation, TNF signaling, COX2, cytokine release and cholesterol/steroid biosynthesis. Down-regulated DEGs reflect losses in cell division cycle (CDC), cyclins (CCN), cyclin-dependent kinases (CDKs), centromere proteins (CENP), kinesin family members (KIFs) and polo-like kinases (PLKs), which were in alignment with biological studies. Conclusion Sub-lethal concentrations of WYE appear to evoke pro-inflammatory, steroid biosynthetic and cytostatic effects in TNBC cells. |
| Databáze: | OpenAIRE |
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