Carbon nanotubes functionalized with folic acid attached via biomimetic peptide linker
| Autor: | Zbigniew Kolacinski, Henryk Wrzosek, Lukasz Szymanski, Ireneusz Majsterek, Karolina Przybylowska-Sygut, Justyna Fraczyk, Malgorzata Walczak, Zbigniew J. Kaminski |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Tetrafluoroborate
Cell Survival Surface Properties Biomedical Engineering Medicine (miscellaneous) Bioengineering Ethylenediamine Peptide 02 engineering and technology Carbon nanotube Development Nanomaterials law.invention Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Folic Acid law Biomimetic Materials Borates Organic chemistry Molecule Humans General Materials Science Particle Size chemistry.chemical_classification Nanotubes Carbon Hydrolysis 021001 nanoscience & nanotechnology Ethylenediamines Combinatorial chemistry chemistry 030220 oncology & carcinogenesis Aminocaproic Acid beta-Alanine 0210 nano-technology Linker HT29 Cells Oligopeptides Conjugate |
| Zdroj: | Nanomedicine (London, England). 12(18) |
| ISSN: | 1748-6963 |
| Popis: | Aim: Anchoring folic acid (FA) with a biomimetic peptidic linker resistant to proteolytic degradation to act as a homing device on functionalized carbon nanotubes. Materials & methods: Ethylenediamine was attached to oxidized multiwalled carbon nanotubes (MWNTs) using 4-(4,6-dimethoxy-[1,3,5]triazin-2-yl)-4-methylmorpholinium tetrafluoroborate. FA was coupled with 6-aminohexanoic acid and derivatives of β-alanine, affording four intermediates, which connected to the MWNTs via peptidic linkers of various lengths. Results: Biomimetic nanomaterials were produced with FA as a homing molecule. The structure and properties of the nanomaterials were analyzed, confirming the versatility of the peptides used as linkers. Conclusion: Conjugates of FA attached to MWNTs via peptide linkers prepared from β-alanine residues are resistant to proteolytic degradation. Viability in colon cancer cells and normal colonocytes confirmed their lack of cytotoxicity. |
| Databáze: | OpenAIRE |
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