Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm

Autor: Kazutoshi Nishijima, Yusuke Sakai, Mai Tanaka, Masatoshi Morimoto, Shinji Yamaguchi, Teruo Watanabe, Jianglin Fan, Shuji Kitajima, Chihiro Koshimoto
Rok vydání: 2014
Předmět:
Zdroj: Experimental Animals
ISSN: 1881-7122
1341-1357
DOI: 10.1538/expanim.63.149
Popis: The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analysis system. Sperm motility with CLC supplementation was 29.4 ± 9.6% (mean ± SD), which was significantly higher than that of controls (20.8 ± 7.1%, P
Databáze: OpenAIRE