Association Between the Lower Extremity Deep Venous Thrombosis, the Warfarin Maintenance Dose, and CYP2C9*3, CYP2D6*10, and CYP3A5*3 Genetic Polymorphisms: A Case-Control Study
Autor: | Yu Gao, Xiaofu Zhang, Feng-Tong Liu, Xin Cao, Cheng-Cheng Yan, Shang Ju |
---|---|
Rok vydání: | 2017 |
Předmět: |
Male
medicine.medical_specialty CYP2D6 030204 cardiovascular system & hematology Polymerase Chain Reaction Polymorphism Single Nucleotide 03 medical and health sciences 0302 clinical medicine Gene Frequency Internal medicine medicine Cytochrome P-450 CYP3A Humans International Normalized Ratio CYP3A5 CYP2C9 Genetics (clinical) Alleles Genetic Association Studies Aged Cytochrome P-450 CYP2C9 Venous Thrombosis biology Dose-Response Relationship Drug Maintenance dose business.industry Warfarin Case-control study Anticoagulants General Medicine Middle Aged medicine.disease CYP2C9*3 Venous thrombosis Cytochrome P-450 CYP2D6 Haplotypes Lower Extremity 030220 oncology & carcinogenesis Anesthesia Case-Control Studies Cardiology biology.protein Female business Polymorphism Restriction Fragment Length medicine.drug |
Zdroj: | Genetic testing and molecular biomarkers. 21(9) |
ISSN: | 1945-0257 |
Popis: | This study explored the association between the CYP2C9*3/CYP2D6*10/CYP3A5*3 genetic polymorphisms with lower extremity deep venous thrombosis (LEDVT) and the warfarin maintenance dose.Five hundred thirty-six patients who were pathologically diagnosed with LEDVT after surgery were included in the LEDVT group. At the same time, 540 patients without LEDVT who underwent surgery were recruited as the control group. Patients were given warfarin at an initial dose of 2.5-3.0 mg. Blood samples were collected to detect the initial and stable international normalized ratio (INR) values. The warfarin maintenance dose was obtained if the INR remained within a range of 2.0-3.0 for 3 consecutive days. The genotype distribution and haplotype analysis of the CYP2C9*3/CYP2D6*10/CYP3A5*3 alleles were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) testing and SHEsis software, respectively. Logistic regression analysis was used to analyze the risk and protective factors for LEDVT.The A/G genotypes, G/G genotypes, and G allele of CYP3A5*3 in the LEDVT group were observed with increased frequency compared with the control group. The LEDVT group displayed a higher ACG haplotype frequency, and lower ACA and ATA haplotype frequencies than the control group. Age, diabetes, low-density lipoprotein, CYP3A5*3 and the ACG haplotype were independent risk factors for LEDVT. High-density lipoprotein and the ACA haplotype were independent protective factors for LEDVT. The genotype distributions of the CYP2C9*3, CYP2D6*10, and CYP3A5*3 genetic polymorphisms were associated with the warfarin maintenance dose.The CYP3A5*3 genetic polymorphism may be an important risk factor for LEDVT. Moreover, CYP2C9*3, CYP2D6*10, and CYP3A5*3 are associated with the warfarin maintenance dose. |
Databáze: | OpenAIRE |
Externí odkaz: |