fost12, the Fusarium oxysporum homolog of the transcription factor Ste12, is upregulated during plant infection and required for virulence
| Autor: | José María Díaz-Mínguez, Brisa Ramos, José J. de Vega-Bartol, Michael H. Perlin, M. Asunción García-Sánchez, Noemí Martín-Rodrigues |
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| Rok vydání: | 2010 |
| Předmět: |
Saccharomyces cerevisiae Proteins
MAP Kinase Signaling System Virulence Factors Molecular Sequence Data Virulence Microbiology Virulence factor Fungal Proteins Gene product Fusarium Gene Expression Regulation Fungal Fusarium oxysporum Gene expression Genetics Amino Acid Sequence DNA Fungal Pathogen Transcription factor Plant Diseases biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Genetic Complementation Test food and beverages Fabaceae Spores Fungal biology.organism_classification Fusarium wilt Up-Regulation Genome Fungal Sequence Alignment Transcription Factors |
| Zdroj: | Fungal Genetics and Biology. 47:216-225 |
| ISSN: | 1087-1845 |
| DOI: | 10.1016/j.fgb.2009.11.006 |
| Popis: | We have identified a Fusarium oxysporum homolog of the Ste12 transcription factor that regulates mating and filamentation in Saccharomyces cerevisiae. The corresponding gene, fost12, from a highly virulent strain of F. oxysporum f. sp. phaseoli, was confirmed to share a high level of similarity and possessed the STE and C2H2 domains characteristic of the fungal Ste12 transcription factor family of proteins. Disruption of fost12 resulted in no visible alterations of colony morphology or in vitro growth characteristics. However, the disruption mutants showed a substantial reduction in virulence when inoculated in common bean seedlings. In planta transcription of fost12 is drastically increased between 12 and 24 h after inoculation, as detected by real-time RT-PCR. The results of the transcriptional analyses carried out in several F. oxysporum strains during axenic growth suggest that the fost12 gene product is a virulence factor required to deal with the nutritional stress confronted by the pathogen during host plant colonization. |
| Databáze: | OpenAIRE |
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