The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose
| Autor: | Maša Ždralević, Zhengchang Liu, Luna Laera, Sergio Giannattasio, Nicoletta Guaragnella, Domenico Marzulli |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death Applied Microbiology Saccharomyces cerevisiae Catabolite repression Biology yeast Biochemistry Genetics and Molecular Biology (miscellaneous) Applied Microbiology and Biotechnology Microbiology mitochondrial retrograde pathway 03 medical and health sciences Virology Genetics Citrate synthase glucose Transcription factor Molecular Biology programmed cell death lcsh:QH301-705.5 Cell Biology Peroxisome biology.organism_classification carbon catabolite repression Yeast Cell biology 030104 developmental biology acetic acid Biochemistry lcsh:Biology (General) Retrograde signaling biology.protein Parasitology |
| Zdroj: | Microbial Cell, Vol 3, Iss 12, Pp 621-631 (2016) Microbial Cell Microbial cell 3 (2016): 490–500. doi:10.15698/mic2016.12.549 info:cnr-pdr/source/autori:Laera, Luna; Guaragnella, Nicoletta; Zdralevic, Masa; Marzulli, Domenico; Liu, Zhengchang; Giannattasio, Sergio/titolo:The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose/doi:10.15698%2Fmic2016.12.549/rivista:Microbial cell/anno:2016/pagina_da:490/pagina_a:500/intervallo_pagine:490–500/volume:3 |
| ISSN: | 2311-2638 |
| Popis: | Yeast Saccharomyces cerevisiae grown on glucose undergoes programmed cell death (PCD) induced by acetic acid (AA-PCD), but evades PCD when grown in raffinose. This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-to-nucleus communication pathway causing up-regulation of various nuclear target genes, such as CIT2, encoding peroxisomal citrate synthase, dependent on the positive regulator RTG2 in response to mitochondrial dysfunction. CCR down-regulates genes mainly involved in mitochondrial respiratory metabolism. In this work, we investigated the relationships between the RTG and CCR pathways in the modulation of AA-PCD sensitivity under glucose repression or de-repression conditions. Yeast single and double mutants lacking RTG2 and/or certain factors regulating carbon source utilization, including MIG1, HXK2, ADR1, CAT8, and HAP4, have been analyzed for their survival and CIT2 expression after acetic acid treatment. ADR1 and CAT8 were identified as positive regulators of RTG-dependent gene transcription. ADR1 and CAT8 interact with RTG2 and with each other in inducing cell resistance to AA-PCD in raffinose and controlling the nature of cell death. In the absence of ADR1 and CAT8, AA-PCD evasion is acquired through activation of an alternative factor/pathway repressed by RTG2, suggesting that RTG2 may play a function in promoting necrotic cell death in repressing conditions when RTG pathway is inactive. Moreover, our data show that simultaneous mitochondrial retrograde pathway activation and SNF1-dependent relief of CCR have a key role in central carbon metabolism reprogramming which modulates the yeast acetic acid-stress response. |
| Databáze: | OpenAIRE |
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