DNA Dendrimers Localize Myod mRNA in Presomitic Tissues of the Chick Embryo
| Autor: | Scott Olex, Robert Getts, Thor W. Nilsen, Harold Weintraub, Christian Lopez, Robert J. Niewenhuis, Mindy George-Weinstein, Michael Baytion, Jacquelyn Gerhart, Steven M DeLuca |
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| Rok vydání: | 2000 |
| Předmět: |
Mesoderm
animal structures Chick Embryo Myosins Biology MyoD 03 medical and health sciences MyoD Protein medicine Animals Tissue Distribution RNA Messenger 030304 developmental biology 0303 health sciences Myogenesis epiblast Muscles 030302 biochemistry & molecular biology Gastrula Cell Biology muscle transcription factor Molecular biology 3. Good health Gastrulation Somite medicine.anatomical_structure Hypoblast Somites Epiblast embryonic structures Original Article segmental plate myogenesis in situ hybridization DNA Probes |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.149.4.825 |
| Popis: | MyoD expression is thought to be induced in somites in response to factors released by surrounding tissues; however, reverse transcription-PCR and cell culture analyses indicate that myogenic cells are present in the embryo before somite formation. Fluorescently labeled DNA dendrimers were used to identify MyoD expressing cells in presomitic tissues in vivo. Subpopulations of MyoD positive cells were found in the segmental plate, epiblast, mesoderm, and hypoblast. Directly after laying, the epiblast of the two layered embryo contained ∼20 MyoD positive cells. These results demonstrate that dendrimers are precise and sensitive reagents for localizing low levels of mRNA in tissue sections and whole embryos, and that cells with myogenic potential are present in the embryo before the initiation of gastrulation. |
| Databáze: | OpenAIRE |
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