First clinical isolate of Escherichia coli harboring mcr-1 gene in Mexico

Autor: Alejandra Aquino-Andrade, Patricia Arzate-Barbosa, Jocelin Merida-Vieyra, Eduardo Arias de la Garza, Alfonso Méndez-Tenorio, Agustín De Colsa Ranero, Jazmin Murcia-Garzón
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Cancer Treatment
Pathology and Laboratory Medicine
medicine.disease_cause
Pediatrics
Database and Informatics Methods
Plasmid
Medicine and Health Sciences
Child
Multidisciplinary
Escherichia coli Proteins
Bacterial Pathogens
Oncology
Experimental Organism Systems
Medical Microbiology
Amikacin
Prokaryotic Models
Medicine
Pathogens
Pediatric Infections
Sequence Analysis
Research Article
medicine.drug
Diarrhea
Escherichia
Virulence Factors
Bioinformatics
Science
030106 microbiology
Virulence
Gastroenterology and Hepatology
Microbial Sensitivity Tests
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Minimum inhibitory concentration
Signs and Symptoms
Model Organisms
Enterobacteriaceae
Diagnostic Medicine
Drug Resistance
Bacterial
Escherichia coli
medicine
Humans
Microbial Pathogens
Mexico
Bacteria
Gut Bacteria
Organisms
Biology and Life Sciences
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
030104 developmental biology
Enteroaggregative Escherichia coli
Animal Studies
Colistin
MCR-1
Zdroj: PLoS ONE, Vol 14, Iss 4, p e0214648 (2019)
PLoS ONE
ISSN: 1932-6203
0000-0000
Popis: Our aim in this report was to describe the characteristics of the first clinical isolate of Escherichia coli (EC-PAG-733) harboring the mcr-1 gene found in Mexico. This isolate was obtained from a fecal sample from a young child with an oncological condition. We obtained the whole-genome sequence using next-generation sequencing and analyzed the sequence by bioinformatics tools. EC-PAG-733 was resistant to third- and fourth-generation cephalosporins and was susceptible to all carbapenems and amikacin; it was also resistant to ciprofloxacin, levofloxacin, gentamicin and colistin at a minimum inhibitory concentration (MIC) of 4 μg/mL. This isolate was classified as O11:H25-ST457. EC-PAG-733 harbored an ESBL type CTX-M-55 as well as several virulence factors that have been associated with Enteroaggregative Escherichia coli (EAEC). The mcr-1 gene was located within an IncI2 plasmid. The results of this whole genome shotgun project were deposited in DDBJ/ENA/GenBank under the accession number QKXE00000000.
Databáze: OpenAIRE
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