Expression of CD95(FAS) by gene transfer does not sensitize K562 to Fas-killing
| Autor: | C. Savary, F. Marini, Michael Andreeff, Reinhold Munker, Shuwei Jiang, L. Owen-Schaub |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Myeloid
Genetic Vectors Cell Culture Techniques chemical and pharmacologic phenomena Apoptosis Biology Natural killer cell Jurkat Cells hemic and lymphatic diseases Leukemia Myelogenous Chronic BCR-ABL Positive medicine Tumor Cells Cultured Humans fas Receptor Annexin A5 CD40 Gene Transfer Techniques hemic and immune systems Hematology Transfection Fas receptor Flow Cytometry Cell biology Killer Cells Natural medicine.anatomical_structure Cell culture Immunology biology.protein Myelopoiesis biological phenomena cell phenomena and immunity Cell Division K562 cells |
| Zdroj: | Hematology and cell therapy. 39(2) |
| ISSN: | 1269-3286 |
| Popis: | CD95(FAS/Apo-1) belongs to the family of TNF receptor related molecules and is expressed on many benign and malignant types of cells. CD95 triggering is involved in the apoptotic death of lymphoid cells and may also be important in myelopoiesis. Myeloid leukemia cells are in most cases resistant to CD95 triggering despite expressing the antigen. We reasoned that myeloid leukemic cells can become sensitized to the Fas pathway of cell death if CD95 expression is restored by gene transfer. As a model, we utilized the cell line K562 which does not express CD95 on their cell surface. The gene for CD95 was introduced into K562 cells (by electroporation). As a control, the gene coding for beta-galactosidase was used. The CD95 transfected K562 cells stably expressed CD95, and had a growth pattern comparable to untransfected cells, but still remained resistant to Fas-triggering (tested using recombinant Fas-ligand). Our experiment shows that antigen expression is not a critical determinant of Fas-mediated apoptosis, but further down-stream mechanisms determine the fate of the cells. The transfected cells also had a comparable susceptibility to NK-mediated lysis which shows that the expression of CD95 does not interfere with NK-mediated lysis of target cells. |
| Databáze: | OpenAIRE |
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