Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae
| Autor: | Patrick R. Murray, Patricia S. Conville, Alexandra Wong, Stephen F. Porcella, Frida Stock, Steven M. Holland, David E. Greenberg, Adrian M. Zelazny |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Acetates Granulomatous Disease Chronic DNA Ribosomal Microbiology Acetobacteraceae Acetic acid chemistry.chemical_compound RNA Ribosomal 16S DNA Ribosomal Spacer Humans Lactic Acid Internal transcribed spacer Acetic acid bacteria Phylogeny Ecology Evolution Behavior and Systematics Base Composition Ethanol biology Methanol Fatty Acids Temperature Genes rRNA Pigments Biological Sequence Analysis DNA General Medicine Hydrogen-Ion Concentration biology.organism_classification 16S ribosomal RNA United States Bacterial Typing Techniques Lactic acid Rec A Recombinases chemistry Biochemistry Granulibacter bethesdensis Oxidation-Reduction Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 56:2609-2616 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A Gram-negative, aerobic, coccobacillus to rod-shaped bacterium was isolated from three patients with chronic granulomatous disease. The organism was subjected to a polyphasic taxonomic study. A multilocus phylogenetic analysis based on the 16S rRNA gene, the internal transcribed spacer (ITS) region and the RecA protein demonstrated that the organism belongs to a new sublineage within the acetic acid bacteria in the familyAcetobacteraceae. Phenotypic features are summarized as follows: the organism grew at an optimum temperature of 35–37 °C and optimum pH of 5.0–6.5. It produced a yellow pigment, oxidized lactate and acetate, the latter weakly, produced little acetic acid from ethanol and could use methanol as a sole carbon source. The two major fatty acids were a straight-chain unsaturated acid (C18 : 1ω7c) and C16 : 0. The DNA base composition was 59.1 mol% G+C. The very weak production of acetic acid from ethanol, the ability to use methanol, the yellow pigmentation and high optimum temperature for growth distinguished this organism from other acetic acid bacteria. The unique phylogenetic and phenotypic characteristics suggest that the bacterium should be classified within a separate genus, for which the nameGranulibacter bethesdensisgen. nov., sp. nov. is proposed. The type strain is CGDNIH1T(=ATCC BAA-1260T=DSM 17861T). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|