Intronic sequences modulate the sensitivity of β-lactoglobulin transgenes to position effects

Autor:	John Webster, Gaetano Donofrio, R M Wallace, Anthony John Clark, Christopher Bruce Alexander Whitelaw
Rok vydání:	1997
Předmět:	biology Transgene Intron Mice Transgenic Lactoglobulins General Medicine Molecular biology Introns In vitro Mice In vivo Genetics biology.protein Transgenic lines Animals Gene silencing Transgenes Gene Beta-lactoglobulin
Zdroj:	Gene. 193:239-243
ISSN:	0378-1119
DOI:	10.1016/s0378-1119(97)00128-5
Popis:	We have analysed the expression of β-lactoglobulin (BLG) gene constructs with combinations of introns deleted to further define the role of intronic regions in directing position-independent mammary expression of BLG transgenes. Intron removal had no obvious effect on hormonal induction of BLG expression in vitro but dramatically reduced expression in vivo, in that removal of intron pairs always resulted in a proportion of the transgenic lines generated failing to express the transgene in the mammary gland. Position-dependent expression was seen for all intron-deleted transgenes regardless of which introns were removed and the ability of the intron-deleted transgenes to be expressed bore no relationship to transgene copy number. Thus, intron removal per se increases the sensitivity of BLG transgenes to position effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::013a46a396a57a5f59b50992d071f227 https://doi.org/10.1016/s0378-1119(97)00128-5 Zobrazit plný text záznamu Full Text from ScienceDirect