Anatoxin-a synthetase gene cluster of the cyanobacterium Anabaena sp. strain 37 and molecular methods to detect potential producers
| Autor: | Muriel Gugger, Anne Rantala-Ylinen, Kaarina Sivonen, Katri A. Berg, Suvi Känä, Ermanno Rizzi, Leo Rouhiainen, Matti Wahlsten, Hao Wang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cyanobacteria
DNA Bacterial Molecular Sequence Data Genetics and Molecular Biology Microcystin Aphanizomenon Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology Anatoxin-a Ligases 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Sequence Homology Nucleic Acid Botany Gene cluster Gene Order 14. Life underwater 030304 developmental biology DNA Primers chemistry.chemical_classification 0303 health sciences Oscillatoria Ecology biology Cyanobacteria Toxins 030306 microbiology Anabaena Sequence Analysis DNA biology.organism_classification 6. Clean water Biosynthetic Pathways chemistry Multigene Family bacteria Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Food Science Biotechnology Tropanes |
| Zdroj: | Applied and environmental microbiology. 77(20) |
| ISSN: | 1098-5336 |
| Popis: | Cyanobacterial mass occurrences are common in fresh and brackish waters. They pose a threat to water users due to toxins frequently produced by the cyanobacterial species present. Anatoxin-a and homoanatoxin-a are neurotoxins synthesized by various cyanobacteria, e.g., Anabaena , Oscillatoria , and Aphanizomenon . The biosynthesis of these toxins and the genes involved in anatoxin production were recently described for Oscillatoria sp. strain PCC 6506 (A. Méjean et al., J. Am. Chem. Soc. 131: 7512-7513, 2009). In this study, we identified the anatoxin synthetase gene cluster ( anaA to anaG and orf1 ; 29 kb) in Anabaena sp. strain 37. The gene (81.6% to 89.2%) and amino acid (78.8% to 86.9%) sequences were highly similar to those of Oscillatoria sp. PCC 6506, while the organization of the genes differed. Molecular detection methods for potential anatoxin-a and homoanatoxin-a producers of the genera Anabaena , Aphanizomenon , and Oscillatoria were developed by designing primers to recognize the anaC gene. Anabaena and Oscillatoria anaC genes were specifically identified in several cyanobacterial strains by PCR. Restriction fragment length polymorphism (RFLP) analysis of the anaC amplicons enabled simultaneous identification of three producer genera: Anabaena , Oscillatoria , and Aphanizomenon . The molecular methods developed in this study revealed the presence of both Anabaena and Oscillatoria as potential anatoxin producers in Finnish fresh waters and the Baltic Sea; they could be applied for surveys of these neurotoxin producers in other aquatic environments. |
| Databáze: | OpenAIRE |
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