Growth inhibition of Candida albicans by interleukin-2-induced lymph node cells
| Autor: | Herbert L. Mathews, David W.A. Beno |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytotoxicity
Immunologic Hyphal growth Time Factors medicine.medical_treatment Lymphocyte Immunology Microbiology Natural killer cell Mice chemistry.chemical_compound Candida albicans medicine Animals Propionibacterium acnes Immunity Cellular Dose-Response Relationship Drug biology Candidiasis biology.organism_classification Corpus albicans Killer Cells Natural Mice Inbred C57BL medicine.anatomical_structure Cytokine chemistry Cell culture Interleukin-2 Female Lymph Nodes Growth inhibition |
| Zdroj: | Cellular Immunology. 128:89-100 |
| ISSN: | 0008-8749 |
| Popis: | Previous reports have demonstrated natural killer cells (NK) to exert growth inhibitory effects against certain fungi, but not against Candida albicans . In this investigation, interleukin-2 (IL-2)-induced lymph node cells with phenotypic and functional characteristics of NK were shown to inhibit the growth of C. albicans . Growth inhibition was evaluated by both the release of 51 Cr by the fungus and the inhibition of microcolony growth of the fungus on Sabouraud's dextrose agar. Lymphoid cells derived from C57B1/6 mice and immediately assessed for hyphal growth inhibition showed little or no activity. However, significant hyphal growth inhibition was produced by lymph node cells cultured with recombinant IL-2. Growth inhibitory activity was dependent upon the concentration of IL-2 and was mediated by nonadherent lymphocytes which lysed an NK-susceptible and to a lesser extent an NK-resistant cell line. Treatment of the IL-2-induced cells with anti-asialo G M1 but not anti-Thy-1 and complement abrogated growth inhibition of C. albicans . These results suggest that IL-2-induced lymph node cells with functional and phenotypic characteristics similar to those of activated NK, mediate in vitro growth inhibition of the hyphal form of C. albicans . |
| Databáze: | OpenAIRE |
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