DECREASED TRABECULAR SMOOTH MUSCLE AND CAVEOLIN-1 EXPRESSION IN THE PENILE TISSUE OF AGED RATS

Autor:	Karl-Dietrich Sievert, Angie Lau, Ching-Shwun Lin, Lora Nunes, Mustafa Emre Bakircioglu, Tom F. Lue
Rok vydání:	2001
Předmět:	Nerve stimulation medicine.medical_specialty medicine.diagnostic_test business.industry Urology Masson's trichrome stain medicine.anatomical_structure Endocrinology Western blot Smooth muscle Latency stage Internal medicine Caveolin 1 Medicine business Immunostaining Penis
Zdroj:	Journal of Urology. 166:734-738
ISSN:	1527-3792 0022-5347
DOI:	10.1016/s0022-5347(05)66052-1
Popis:	Purpose: Because decreased trabecular smooth muscle content is reportedly associated with vasculogenic impotence in men, we performed a rodent study to investigate the effect of aging on trabecular smooth muscle content and caveolin-1 protein expression in penile smooth muscle cells.Materials and Methods: In 6 young (age 3 months) and 6 old (age 24 months) rats erectile function was evaluated by cavernous nerve stimulation. At sacrifice penile tissue samples were collected for Western blot analysis, Masson’s trichrome staining, caveolin-1 immunostaining and electron microscopy. The percent of smooth muscle in the trabecular tissue was assessed by computer assisted image analysis.Results: In the aged rats mean intracavernous pressure plus or minus standard deviation was decreased (70 ± 8.8 versus 107 ± 12.3 cm. water) and the latency period was increased (7.8 ± 1.2 versus 4.5 ± 0.5 seconds) significantly compared to values in the young rats (p
Databáze:	OpenAIRE
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