Transfecting well-differentiated prostatic cancer cell line LNCaP
| Autor: | Minna Ruokonen, Pirjo Hedberg, Pirkko Vihko, Jingdong Shan, Lila Patrikainen |
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| Rok vydání: | 1996 |
| Předmět: |
Male
animal structures viruses Genetic Vectors Biophysics Cytomegalovirus Cytomegalovirus promoter Biology Transfection Biochemistry Plasmid LNCaP Tumor Cells Cultured Humans Promoter Regions Genetic Molecular Biology fungi Prostatic Neoplasms Cell Differentiation Cell Biology Serum concentration Molecular biology Well differentiated embryonic structures Cancer cell lines |
| Zdroj: | Biochemical and biophysical research communications. 218(3) |
| ISSN: | 0006-291X |
| Popis: | The transfection of well-differentiated sensitive cells requires careful optimization of the conditions used. In addition to the transfection method chosen, the amount of cells plated, and thus the density of the cells, as well as the influence of the serum concentration play a critical role in the case of LNCaP cells. We also found out that the only appropriate control plasmid for the transfection efficiency was pCMVβ-gal driven by the cytomegalovirus promoter. |
| Databáze: | OpenAIRE |
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