Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals
| Autor: | Marga Van Santen-Verheuvel, Christina M. J. E. Vandenbroucke-Grauls, Rob J. L. Willems, David Tribe, Eric van Kregten, Xenia Manzioros, Ellen M. Mascini, Marc J. M. Bonten, Carlo A. J. M. Gaillard, Janetta Top, Jan D. A. van Embden, Wieger L. Homan |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Bacterial Genetic Markers Genotype Enterococcus faecium Virulence Microbiology Bacterial Proteins Vancomycin medicine Humans Phylogeny Antibacterial agent Cross Infection biology business.industry Outbreak Membrane Proteins Vancomycin Resistance General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Carriage Genetic marker business Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Lancet (London, England). 357(9259) |
| ISSN: | 0140-6736 |
| Popis: | In the USA, vancomycin-resistant Enterococcus faecium (VREF) is endemic in hospitals, despite lack of carriage among healthy individuals. In Europe, however, hospital outbreaks are rare, but VREF carriage among healthy individuals and livestock is common. We used amplified fragment-length polymorphism analysis to genotype 120 VREF isolates associated with hospital outbreaks and 45 non-epidemic isolates from the USA, Europe, and Australia. We also looked for the esp virulence gene in these isolates and in 98 VREF from animals. A specific E. faecium subpopulation genetically distinct from non-epidemic VREF isolates was found to be the cause of the hospital epidemics in all three continents. This subpopulation contained a variant of the esp gene that was absent in all non-epidemic and animal isolates. Identification of the variant esp gene will be important in guiding infection-control strategies, and the Esp protein could be a new target for antibacterial therapy. |
| Databáze: | OpenAIRE |
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