Resident Renal Mononuclear Phagocytes Comprise Five Discrete Populations with Distinct Phenotypes and Functions
| Autor: | Steven F. Ziegler, Lucas J. Thompson, Julia Lichtnekert, Tobias M. Hohl, Jeremy S. Duffield, Peter J. Nelson, Jay W. Heinecke, Hicham Bouabe, Prasanthi Karna, Takahisa Kawakami |
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| Rok vydání: | 2013 |
| Předmět: |
T-Lymphocytes
CD14 medicine.medical_treatment Immunology CD11c CD16 Kidney Article Mice medicine Animals Immunology and Allergy Macrophage Mononuclear Phagocyte System Tissue homeostasis Mice Inbred BALB C biology Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Flow Cytometry Phenotype Mice Inbred C57BL Cytokine Microscopy Fluorescence Integrin alpha M biology.protein |
| Zdroj: | The Journal of Immunology. 191:3358-3372 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Recent reports have highlighted greater complexity, plasticity, and functional diversity of mononuclear phagocytes (MPCs), including monocytes, macrophages, and dendritic cells (DCs), in our organs than previously understood. The functions and origins of MPCs resident within healthy organs, especially in the kidney, are less well understood, whereas studies suggest they play roles in disease states distinct from recruited monocytes. We developed an unbiased approach using flow cytometry to analyze MPCs residing in the normal mouse kidney, and identified five discrete subpopulations according to CD11b/CD11c expression as well as F4/80, CD103, CD14, CD16, and CD64 expression. In addition to distinct marker profiles, these subpopulations have different lineages and expression of genes involved in tissue homeostasis, including angiogenesis. Among them, the CD11bintCD11cint F4/80high subpopulation notably exhibited high capacity to produce a representative anti-inflammatory cytokine, IL-10. Each subpopulation had different degrees of both macrophage (phagocytosis) and DC (Ag presentation) capacities, with a tendency to promote differentiation of regulatory T cells, whereas two of these showed expression of transcription factors reported to be highly expressed by classical DCs, and proclivity to exit the kidney following stimulation with LPS. In summary, resident kidney MPCs comprise discrete subpopulations, which cannot be simply classified into the conventional entities, and they produce anti-inflammatory and tissue-homeostatic factors to differing degrees. |
| Databáze: | OpenAIRE |
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