There are Abundant Antimicrobial Peptides in Brains of Two Kinds ofBombinaToads
| Autor: | Ren Lai, Huahu Ye, Jing Wu, Hailong Yang, Huan Liu, Rui Liu, Yufang Ma |
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| Rok vydání: | 2011 |
| Předmět: |
Molecular Sequence Data
Antimicrobial peptides Peptide Microbial Sensitivity Tests Biology Bombina maxima Hemolysis Biochemistry Mass Spectrometry Microbiology Anti-Infective Agents Complementary DNA Animals Amino Acid Sequence Phylogeny Brain Chemistry Cloning chemistry.chemical_classification Base Sequence cDNA library General Chemistry Antimicrobial biology.organism_classification chemistry Anura Bacteria Antimicrobial Cationic Peptides |
| Zdroj: | Journal of Proteome Research. 10:1806-1815 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr101285n |
| Popis: | It is well-known that there is a large amount of antimicrobial peptides in amphibian skins but few antimicrobial peptides are found in amphibian brains. Twenty-two and four antimicrobial peptides were purified and characterized from the brain homogenate of Bombina maxima and B. microdeladigitora, respectively. One hundred fifty-eight cDNA clones encoding 79 antimicrobial peptides were isolated from brain cDNA libraries of B. maxima and B. microdeladigitora. These antimicrobial peptides belong to two peptide groups (maximin and maximin-H). Twenty of them are identical to previously reported antimicrobial peptides (maximin 1-8, 10, 11, maximin H1, 3-5, 7, 9, 10, 12, 15, 16) from B. maxima skin secretions. Fifty-nine of them are novel antimicrobial peptides. Some of these antimicrobial peptides showed strong antimicrobial activities against tested microorganism strains including Gram-positive and -negative bacteria and fungi. The current diversity in peptide coding cDNA sequences is, to our knowledge, the most extreme yet described for any animal brains. The extreme diversity may give rise to interest to prospect the actual functions of antimicrobial peptides in amphibian brains. |
| Databáze: | OpenAIRE |
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