Inhibition of oxidative phosphorylation, vascular tone, and [Ca2+]i in the perfused rat tail artery
| Autor: | Jeffrey Atkinson, Nguyen Tran, C. Capdeville-Atkinson, A. Robert |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Vascular smooth muscle Fura-2 Physiology chemistry.chemical_element Oxidative phosphorylation In Vitro Techniques Biology Calcium Muscle Smooth Vascular Oxidative Phosphorylation Calcium in biology Potassium Chloride Norepinephrine chemistry.chemical_compound Adenosine Triphosphate Sodium Cyanide medicine Animals Rats Wistar Sodium cyanide Arteries Cell Biology Rats Kinetics Biochemistry chemistry Vasoconstriction Muscle Tonus Biophysics medicine.symptom Dinitrophenols Intracellular |
| Zdroj: | American Journal of Physiology-Cell Physiology. 273:C834-C842 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1997.273.3.c834 |
| Popis: | We investigated the possibility that the inhibition of oxidative phosphorylation in vascular smooth muscle attenuates norepinephrine- or KCl-evoked vasoconstriction with no change in mobilization of intracellular calcium concentration ([Ca2+]i). Experiments were performed in perfused segments of the rat tail artery loaded with the intracellular calcium dye fura 2, in the absence and presence of dinitrophenol or sodium cyanide; inhibition of oxidative phosphorylation was evaluated from the fall in intracellular ATP levels. The metabolic inhibitors reduced vasoconstriction with no change in [Ca2+]i handling, suggesting that 1) inhibition of oxidative phosphorylation attenuates vasoconstriction via a mechanism downstream of [Ca2+]i, and 2) [Ca2+]i homeostasis (both increases and decreases in [Ca2+]i) can be maintained in the presence of inhibitors of oxidative phosphorylation. |
| Databáze: | OpenAIRE |
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