Recombinant IFN-alpha2b treatment activates poly (ADPR) polymerase-1 (PARP-1) in KB cancer cells
| Autor: | Piera Quesada, S. Di Meglio, Antonietta Fabbrocini, Stefano Pepe, Corrado Garbi, Ar Bianco, M. Malanga, S. De Lorenzo |
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| Přispěvatelé: | Quesada, PIERINA MARIA, Malanga, Maria, DI MEGLIO, Silvia, DE LORENZO, Sonya, Fabbrocini, Antonietta, Garbi, Corrado, Bianco, ANGELO RAFFAELE, Pepe, Stefano |
| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
KB cell line Poly ADP ribose polymerase Blotting Western Fluorescent Antibody Technique Antineoplastic Agents Biology Interferon alpha-2 IFN KB Cells chemistry.chemical_compound PARP1 inhibition Tumor Cells Cultured Humans cell growth inhibition Cell growth Cell Cycle Interferon-alpha Flow Cytometry Molecular biology apoptosi flow cytometry Recombinant Proteins Enzyme Activation Oncology chemistry Biochemistry Epidermoid carcinoma 3-Aminobenzamide Cell culture Apoptosis Cancer cell Carcinoma Squamous Cell Growth inhibition Poly(ADP-ribose) Polymerases Cell Division |
| Zdroj: | European journal of cancer (Oxford, England : 1990). 39(14) |
| ISSN: | 0959-8049 |
| Popis: | In the present paper, we investigated the relationship between the growth inhibitory effects of recombinant interferon-alpha2b (rIFN-alpha2b) and poly (ADPR) polymerase-1 (PARP-1) activity in the human squamous KB cancer cell line. Growth inhibition of the KB cells mediated by 1000 IU/ml of rIFN-alpha2b was accompanied by a transient rise in PARP-1 specific activity 24 h after rIFN-alpha2b treatment, confirmed by both the increase of intracellular poly (ADP-ribose) content and the PARP-1 auto-modification level. At longer times of incubation, the onset of apoptosis accompanied KB cell growth inhibition, as demonstrated by both flow cytometry and western-blotting analysis showing an 89 kDa apoptotic fragment of PARP-1. Moreover, pretreatment of the cells with the PARP-1 inhibitor, 3-aminobenzamide (3-ABA), at non-cytotoxic concentrations (1 mM), reduced the cell-growth inhibition, cell-cycle perturbation and apoptosis caused by rIFN-alpha2b. Taken together, these results strongly suggest that PARP-1 may be directly involved in the effects of rIFN-alpha2b in the KB cancer cell line. |
| Databáze: | OpenAIRE |
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