Trypanosoma evansi: concentration of 3-nitrotyrosine in the brain of infected rats
| Autor: | Aleksandro Schafer da Silva, Carlos Breno Paim, Márcio Machado Costa, Silvia Gonzalez Monteiro, Luiz Fernando Almeida Silva, Carlos Fernando Mello, Mauro Schneider Oliveira, Cinthia M. Mazzanti, Sonia Terezinha dos Anjos Lopes, Patrícia Wolkmer, Cássia B. da Silva, Francine C. Paim |
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| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Inflammatory processes medicine.medical_treatment Central nervous system Immunology Dot blot Parasitemia Hippocampus Nitric oxide Andrology chemistry.chemical_compound Dogs Trypanosomiasis Typanosomosis Cerebellum medicine Hippocampus (mythology) Animals Rats Wistar Saline Cerebral Cortex biology Brain General Medicine Trypanosoma evansi medicine.disease biology.organism_classification Corpus Striatum Rats Infectious Diseases medicine.anatomical_structure chemistry Isoflurane Case-Control Studies Tyrosine Parasitology CNS medicine.drug |
| Zdroj: | Experimental parasitology. 129(1) |
| ISSN: | 1090-2449 |
| Popis: | Nitric oxide (NO) is involved in many physiological processes, such as blood pressure control, neurotransmission, inhibition of platelet and neutrophil adherence, and the ability to kill tumor cells and parasites. The indirect determination of NO can be made by detection of 3-nitrotyrosine (3-NT) residues. The aim of this study was to measure the concentration of 3-NT in the brain of rats experimentally infected with Trypanosoma evansi. Twenty-four were inoculated intraperitoneally with cryopreserved blood containing 1×106 trypomastigotes per animal. Twenty-four animals were used as negative controls and received 0.2mL of saline by the same route. The experimental groups (group C and T) were established according to the time after infection and the degree of parasitemia as follows: four control subgroups (C3, C5, C10 and C20) with six non-inoculated animals each and four test subgroups (T3, T5, T10 and T20) with six animals infected with T. evansi in each group. The animals were anesthetized with isoflurane and subsequently euthanized at the days 3 (C3, T3), 5 (C5, T5), 10 (C10, T10) and 20 (C20, T20) post-infection (PI). The brain was removed and dissected into cerebellum, cerebral cortex, striatum and hippocampus. Concentration of 3-NT in the brain was determined by Slot blot technique. At the day 3 PI no changes were observed in the concentration of 3-NT among the groups. There was a significant reduction (p |
| Databáze: | OpenAIRE |
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