A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

Autor:	Stefanie Brands, Andreas Sebastian Klein, Anna Joëlle Ruff, Hannah U. C. Brass, Jörg Pietruszka, Ulrich Schwaneberg
Rok vydání:	2020
Předmět:	0301 basic medicine High-throughput screening 01 natural sciences Catalysis Substrate Specificity Ligases Prodigiosin 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Escherichia coli Materials Chemistry Plate assay chemistry.chemical_classification DNA ligase biology Pseudomonas putida 010405 organic chemistry Metals and Alloys General Chemistry Prodiginine Directed evolution biology.organism_classification High-Throughput Screening Assays 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Kinetics 030104 developmental biology chemistry Biochemistry ddc:540 Mutagenesis Site-Directed Ceramics and Composites Colorimetry Directed Molecular Evolution
Zdroj:	Chemical communications 56(61), 8631-8634 (2020). doi:10.1039/D0CC02181D
ISSN:	1364-548X 1359-7345
Popis:	A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::012c6bc42ca8f04f4aeeaaefe2adfe51 https://doi.org/10.1039/d0cc02181d Zobrazit plný text záznamu