Supercritical fluid extraction and reversed-phase liquid chromatography methods for vitamin A and β-carotene heterogeneous distribution of vitamin A in the liver
| Autor: | Monica R. Wong, Terry R. Neidlinger, Annie O. Lo, Cynthia Kwan, Betty J. Burri |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Vitamin
medicine.medical_treatment Biochemistry High-performance liquid chromatography Antioxidants Analytical Chemistry chemistry.chemical_compound medicine Animals Vitamin A Chromatography High Pressure Liquid Chromatography Organic Chemistry Carotene Supercritical fluid extraction Retinol Reproducibility of Results General Medicine Reversed-phase chromatography beta Carotene Supercritical fluid Liver chemistry Cattle Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 762:201-206 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(96)00705-4 |
| Popis: | We developed supercritical fluid procedures for extracting vitamin A and beta-carotene from vitamin supplements and calf liver tissue. The SF extracts could be injected onto an HPLC column without further pretreatment. Samples were analysed by RP-HPLC using diode array detection or by spectrophotometry. Recoveries were very good. SF extracts from a vitamin preparation of uniform composition had an R.S.D. of 4%. Extracts from calf liver supplements were predictably more heterogeneous. The SF extraction method is less labor intensive then traditional liquid-liquid procedures for extracting vitamin A and carotenoids from tissues. |
| Databáze: | OpenAIRE |
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