Investigation of the Fate and Dynamics of Extracellular Polymeric Substances (EPS) during Sludge-Based Photogranulation under Hydrostatic Conditions
| Autor: | Anastasia Ivanova, Kristie Stauch-White, Sona Dolan, Gitau J. Gikonyo, Wenye Camilla Kuo-Dahab, Chul Park, Caitlyn S. Butler, Blanca I. Carbajal-González |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chlorophyll a Sewage Chemistry Extracellular Polymeric Substance Matrix Chlorophyll A Biomass General Chemistry 010501 environmental sciences Wastewater 01 natural sciences Bioavailability 03 medical and health sciences chemistry.chemical_compound Granulation 030104 developmental biology Activated sludge Extracellular polymeric substance Environmental chemistry Environmental Chemistry Aeration 0105 earth and related environmental sciences |
| Zdroj: | Environmental sciencetechnology. 52(18) |
| ISSN: | 1520-5851 |
| Popis: | Oxygenic photogranules have received increasing interest due to their ability to treat wastewater without aeration and recover wastewater's chemical energy and solar energy. It has been reported that these photogranules can be produced under both hydrostatic and hydrodynamic conditions, and enrichment of filamentous cyanobacteria is required for this photogranulation to occur. Despite the critical role extracellular polymeric substances (EPS) play in granulation, EPS in photogranulation is yet virtually unknown. Here, we present the fate and dynamics of different fractions of EPS in sludge-based photogranulation under hydrostatic conditions. The study shows that during the transformation of activated sludge into a photogranular biomass, sludge's base-extractable proteins selectively degrade. Strong correlations between base-extracted proteins and the growth of chlorophyll a and chlorophyll a/ b ratio suggest that the bioavailability of this organic nitrogen is linked with selection and enrichment of filamentous cyanobacteria under hydrostatic conditions. The results of soluble and sonication-extractable EPS and microscopy also show that the growth of filamentous cyanobacteria required large amounts of polysaccharide-based EPS for their motility and maintenance. With findings on the progression of photogranulation, the fate and dynamics of EPS, and microscopy on microstructures associated with EPS, we discuss potential mechanisms of photogranulation occurring under hydrostatic conditions. |
| Databáze: | OpenAIRE |
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