Transcranial low‐level laser therapy in an in vivo model of stroke: Relevance to the brain infarct, microglia activation and neuroinflammation
| Autor: | Flavio Aimbire, Nancy N. Ortiz-Villatoro, Marcia Jonathas Guimaraes Marques, Debora D.S. Vogel, Fulvio A. Scorza, Regiane Albertini, Noemi S. Araújo, Carla A. Scorza |
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| Rok vydání: | 2021 |
| Předmět: |
Brain Infarction
Pathology medicine.medical_specialty Ischemia General Physics and Astronomy Brain damage 01 natural sciences Neuroprotection General Biochemistry Genetics and Molecular Biology 010309 optics In vivo 0103 physical sciences medicine Animals Humans General Materials Science Low-Level Light Therapy Rats Wistar Stroke Neuroinflammation Microglia biology business.industry 010401 analytical chemistry General Engineering General Chemistry medicine.disease Rats 0104 chemical sciences medicine.anatomical_structure biology.protein NeuN medicine.symptom business |
| Zdroj: | Journal of Biophotonics. 14 |
| ISSN: | 1864-0648 1864-063X |
| Popis: | Stroke is the main cause of death and functional disability. The available therapy affects only 5% of patients, and new therapeutic approaches have been constantly tested. Transcranial photobiomodulation (PBM) is promising for its neuroprotective effect on brain injuries. Thus, the present study investigated the PBM effects in an in vivo model of ischemic stroke induced by photothrombosis (PT). Five different groups of Wistar rats were submitted or not to a daily dose of fish oil or/and laser sessions for 2 months. The ischemia volume was evaluated by stereology; GFAP, Iba, and NeuN by immunohistochemistry; TNF-α, IL-1β, IL-6, IL-10, and TGF-β by ELISA assay. PBM influenced both the lesion volume and the GFAP. Furthermore, PBM and Ω-3 or both reduced Iba RNAm. PBM reduced TNF-α, IL-1β, IL-6, brain damage, neuroinflammation, and microglial activation, and it increased astroglial activity in peri-lesioned region after stroke. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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