Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients
| Autor: | Corinne Houles, Anne Imberty, Edward P. Mitchell, Serge Pérez, Michaela Wimmerová, Catherine Gautier, Dvora Sudakevitz, Albert M. Wu, Nechama Gilboa-Garber |
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| Přispěvatelé: | Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular Cystic Fibrosis Macromolecular Substances Oligosaccharides Fucose binding Biology Crystallography X-Ray medicine.disease_cause Biochemistry Bacterial Adhesion Fucose Microbiology chemistry.chemical_compound Structural Biology Lectins Genetics medicine Humans Binding site Adhesins Bacterial Lung ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Binding Sites Pseudomonas aeruginosa Lectin Oligosaccharide Bacterial adhesin chemistry Docking (molecular) biology.protein Calcium Protein Binding |
| Zdroj: | Nat. Struct. Biol. Nat. Struct. Biol., 2002, pp.918-921 |
| ISSN: | 1072-8368 |
| DOI: | 10.1038/nsb865 |
| Popis: | Pseudomonas aeruginosa galactose- and fucose-binding lectins (PA-IL and PA-IIL) contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL in complex with fucose reveals a tetrameric structure. Each monomer displays a nine-stranded, antiparallel b-sandwich arrangement and contains two close calcium cations that mediate the binding of fucose in a recognition mode unique among carbohydrate-protein interactions. Experimental binding studies, together with theoretical docking of fucose-containing oligosaccharides, are consistent with the assumption that antigens of the Lewis a (Le(a)) series may be the preferred ligands of this lectin. Precise knowledge of the lectin-binding site should allow a better design of new antibacterial-adhesion prophylactics. |
| Databáze: | OpenAIRE |
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