Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells
| Autor: | Yun‑Min Lu, Yuan‑Fei Liu, Yuan Liu, Xiao‑Hong Liao, Wu‑Ming Kong, Guo‑Qiang Qu, Wei-Xiong Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Cancer Research Apoptosis Biochemistry Small hairpin RNA 0302 clinical medicine RNA interference RNA Small Interfering Notch signaling bcl-2-Associated X Protein Aged 80 and over biology Receptors Notch Intracellular Signaling Peptides and Proteins Articles Cell cycle Middle Aged Cell biology Gene Expression Regulation Neoplastic Oncology 030220 oncology & carcinogenesis Gene Knockdown Techniques Molecular Medicine Female RNA Interference Signal transduction Signal Transduction Adult Down-Regulation Protein Serine-Threonine Kinases 03 medical and health sciences Bcl-2-associated X protein Stomach Neoplasms Cell Line Tumor Genetics Humans Neoplasm Invasiveness Gene Silencing RNA Messenger Protein kinase A Molecular Biology Aged Cell Proliferation G1 arrest Cell growth gastric cancer G1 Phase Cell Cycle Checkpoints 030104 developmental biology Cancer cell biology.protein Cancer research MAP4K4 |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Gastric cancer (GC) is the second most common cause of cancer-associated mortality worldwide. Previous studies suggest that mitogen-activated protein kinase kinase kinase kinase isoform 4 (MAP4K4) is involved in cancer cell growth, apoptosis and migration. In the present study, bioinformatics analysis and reverse transcription-quantitative polymerase chain reaction were performed to determine if MAP4K4 was overexpressed in GC. The knockdown of MAP4K4 by RNA interference in GC cells markedly inhibited cell proliferation, which may be mediated by cell cycle arrest in the G1 phase. The silencing of MAP4K4 also induced cell apoptosis by increasing the ratio of Bax/Bcl-2. In addition, Notch signaling was markedly reduced by MAP4K4 silencing. The results of the present study suggested that inhibition of MAP4K4 may be a therapeutic strategy for GC. |
| Databáze: | OpenAIRE |
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