Insulin and GSK3β-inhibition abrogates the infarct sparing-effect of ischemic postconditioning in ex vivo rat hearts

Autor: Lars Breivik, Erik Helgeland, Anne K. Jonassen, Rune Kringstad Sandøy, Anne Aspevik, Anita Wergeland, Maren Haave Askeland
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
medicine.medical_treatment
Myocardial Infarction
Endogeny
030204 cardiovascular system & hematology
Pharmacology
Aminophenols
STAT3
Maleimides
0302 clinical medicine
Insulin
GSK3β inhibition
Myocardial infarction
Phosphorylation
Ischemic Postconditioning
ischemia reperfusion injury
Cardioprotection
Heart
reperfusion
Cardiology
Cardiology and Cardiovascular Medicine
Signal Transduction
STAT3 Transcription Factor
insulin
medicine.medical_specialty
Ischemia
Myocardial Reperfusion Injury
03 medical and health sciences
Internal medicine
medicine
Animals
ischemic postconditioning
Rats
Wistar
Protein kinase B
Protein Kinase Inhibitors
Glycogen Synthase Kinase 3 beta
business.industry
Akt
Myocardium
GSK3β
Isolated Heart Preparation
medicine.disease
Disease Models
Animal
030104 developmental biology
business
Reperfusion injury
Proto-Oncogene Proteins c-akt
Ex vivo
Zdroj: Scandinavian Cardiovascular Journal
ISSN: 1651-2006
1401-7431
Popis: Objectives. Pharmacological treatment of reperfusion injury using insulin and GSK3β inhibition has been shown to be cardioprotective, however, their interaction with the endogenous cardioprotective strategy, ischemic postconditioning, is not known. Design. Langendorff perfused ex vivo rat hearts were subjected to 30 min of regional ischemia and 120 min of reperfusion. For the first 15 min of reperfusion hearts received either vehicle (Ctr), insulin (Ins) or a GSK3β inhibitor (SB415286; SB41), with or without interruption of ischemic postconditioning (IPost; 3 × 30 s of global ischemia). In addition, the combination of insulin and SB41 for 15 min was assessed. Results. Insulin, SB41 or IPost significantly reduced infarct size versus vehicle treated controls (IPost 33.5 ± 3.3%, Ins 33.5 ± 3.4%, SB41 30.5 ± 3.0% vs. Ctr 54.7 ± 6.8%, p
Databáze: OpenAIRE
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