| Autor: |
Thomas Moritz, Ulrich Dührsen, Michael Flasshove, Siegfried Seeber, Ludger Sellmann, Joachim Göthert, Philipp Schütt, Michael Möllmann, Ursula R. Sorg, Florian Grabellus, Peter Ebeling, Jan Dürig |
| Rok vydání: |
2023 |
| Popis: |
We here describe a novel xenograft model of chronic lymphocytic leukemia (CLL) generated by infusion of human primary CLL cells into immunodeficient nonobese/severe combined immunodeficient (NOD/SCID) mice. Combined i.v. and i.p. injection of peripheral blood mononuclear cells (PBMC) from 39 patients with CLL resulted in highly reproducible splenic (37 of 39) and peritoneal (35 of 39) engraftment, which remained stable over a time span of 4 to 8 weeks. By comparison, recovery of leukemic cells from bone marrow (21 of 39) or peripheral blood (8 of 22) was substantially lower. The engraftment pattern of CLL PBMC 4 weeks posttransplant was correlated with clinical disease activity: infusion of PBMC from donors with Binet stage A, lymphocyte doubling time of >12 months, and normal lactate dehydrogenase (LDH) serum levels led to marked engraftment of T cells whereas comparably few tumor cells could be detected. In contrast, NOD/SCID mice receiving PBMC from donors with advanced stage Binet C, lymphocyte doubling time of |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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