Neuronostatin inhibits glucose-stimulated insulin secretion via direct action on the pancreatic α-cell
| Autor: | John A. Corbett, Willis K. Samson, Gina L. C. Yosten, Alison S. Salvatori, Mollisa M. Elrick |
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| Rok vydání: | 2014 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Inositol 1 4 5-Trisphosphate Peptide hormone Biology Bradykinin Real-Time Polymerase Chain Reaction Glucagon Cell Line Rats Sprague-Dawley Islets of Langerhans Physiology (medical) Internal medicine medicine Cyclic AMP Glucose homeostasis Animals Insulin geography geography.geographical_feature_category Pancreatic islets Articles Islet Cyclic AMP-Dependent Protein Kinases Peptide Fragments Rats medicine.anatomical_structure Somatostatin Endocrinology Glucose Glucagon-Secreting Cells Area Under Curve Electrophoresis Polyacrylamide Gel Pancreas Injections Intraperitoneal |
| Zdroj: | American journal of physiology. Endocrinology and metabolism. 306(11) |
| ISSN: | 1522-1555 |
| Popis: | Neuronostatin is a recently described peptide hormone encoded by the somatostatin gene. We previously showed that intraperitoneal injection of neuronostatin into mice resulted in c-Jun accumulation in pancreatic islets in a pattern consistent with the activation of glucagon-producing α-cells. We therefore hypothesized that neuronostatin could influence glucose homeostasis via a direct effect on the α-cell. Neuronostatin enhanced low-glucose-induced glucagon release in isolated rat islets and in the immortalized α-cell line αTC1-9. Furthermore, incubation with neuronostatin led to an increase in transcription of glucagon mRNA, as determined by RT-PCR. Neuronostatin also inhibited glucose-stimulated insulin secretion from isolated islets. However, neuronostatin did not alter insulin release from the β-cell line INS 832/13, indicating that the effect of neuronostatin on insulin secretion may be secondary to a direct action on the α-cell. In agreement with our in vitro data, intra-arterial infusion of neuronostatin in male rats delayed glucose disposal and inhibited insulin release during a glucose challenge. These studies suggest that neuronostatin participates in maintaining glucose homeostasis through cell-cell interactions between α-cells and β-cells in the endocrine pancreas, leading to attenuation in insulin secretion. |
| Databáze: | OpenAIRE |
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