3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices
| Autor: | Arto Heiskanen, Roger A. Barker, Malin Parmar, Alberto Martínez-Serrano, Sebastian Buchmann, Jenny Emnéus, Janko Kajtez, Shashank Vasudevan, Marcella Birtele, Johan Ulrik Lind, Christian Jonathan Pless, Stefano Rocchetti |
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| Přispěvatelé: | European Commission, Lund University, Kajtez, Janko [0000-0001-9997-2325], Vasudevan, Shashank [0000-0001-6490-3434], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
3d printed
fast prototyping Computer science General Chemical Engineering Science Human neural stem cells Microfluidics neurite guidance General Physics and Astronomy Medicine (miscellaneous) 3D printing soft lithography Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Corrections Soft lithography In vitro model compartmentalized devices SDG 3 - Good Health and Well-being General Materials Science Neurite guidance lcsh:Science nigrostriatal pathway Nigrostriatal pathway Full Paper business.industry General Engineering Compartmentalized devices Correction Full Papers 021001 nanoscience & nanotechnology 0104 chemical sciences Fast prototyping human neural stem cells lcsh:Q Neuroscience research 0210 nano-technology business |
| Zdroj: | Kajtez, J, Buchmann, S, Vasudevan, S, Birtele, M, Rocchetti, S, Pless, C J, Heiskanen, A, Barker, R A, Martínez-Serrano, A, Parmar, M, Lind, J U & Emnéus, J 2020, ' 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices ', Advanced Science, vol. 7, no. 16, 2001150 . https://doi.org/10.1002/advs.202001150 Advanced Science Advanced Science, Vol 7, Iss 16, Pp n/a-n/a (2020) Advanced Science, Vol 8, Iss 12, Pp n/a-n/a (2021) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high‐aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open‐well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long‐term maintenance of healthy human stem‐cell derived neurons and astrocytes, spanning at least 40 days. Leveraging fast‐prototyping capabilities at both micro and macroscale, a proof‐of‐principle human in vitro model of the nigrostriatal pathway is created. By presenting a route for novel materials and unique architectures in microfluidic systems, the method provides new possibilities in biological research beyond neuroscience applications. In this study, a hybrid additive manufacturing approach to soft lithography is developed for the fabrication of open‐well compartmentalized microfluidic devices used to engineer human stem‐cell derived neural networks in vitro. The approach provides larger freedom of design, removes the need for manual postprocessing, increases the biocompatibility of the system, and enables fast prototyping at the micro and macroscale. |
| Databáze: | OpenAIRE |
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