Protective effect of a Butea monosperma (Lam.) Taub. flowers extract against skin inflammation: Antioxidant, anti-inflammatory and matrix metalloproteinases inhibitory activities
Autor: | Claire Elfakir, Emilie Destandau, Thomas Michel, Isabelle Krolikiewicz-Renimel, Patrice Andre, Chantal Pichon, Madhukar Reddy |
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Přispěvatelé: | Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunobiologie fondamentale et clinique, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO)-Institut de Chimie du CNRS (INC), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2013 |
Předmět: |
Adult
Keratinocytes Antioxidant medicine.drug_class [SDV]Life Sciences [q-bio] medicine.medical_treatment Anti-Inflammatory Agents Dermatitis Inflammation Flowers Matrix metalloproteinase Antioxidants Dinoprostone Anti-inflammatory 03 medical and health sciences Chalcones 0302 clinical medicine Butea Drug Discovery medicine Humans Prostaglandin E2 Cells Cultured Skin 030304 developmental biology Flavonoids 2. Zero hunger Pharmacology 0303 health sciences Traditional medicine biology Plant Extracts Chemistry biology.organism_classification Matrix Metalloproteinases In vitro 3. Good health Cell culture 030220 oncology & carcinogenesis Cytokines Female Epidermis medicine.symptom medicine.drug |
Zdroj: | Journal of Ethnopharmacology Journal of Ethnopharmacology, Elsevier, 2013, 148 (2), pp.537-543. ⟨10.1016/j.jep.2013.05.001⟩ Journal of Ethnopharmacology, 2013, 148 (2), pp.537-543. ⟨10.1016/j.jep.2013.05.001⟩ |
ISSN: | 0378-8741 |
Popis: | International audience; Butea monosperma (Lam.) Taubert (Syn. Butea frondosa; family Fabaceae) is a common plant of the Indian continent (Das et al., 2011; Sharma and Deshwal, 2011). The brightly orange flowers of this plant are widely used in traditional medicine and more particularly for inflammatory disease.AIM OF THE STUDY:In vitro anti-inflammatory mechanism of a hydroethanolic extract of B. monosperma flowers (BME) and more specifically of an enriched fraction in butrin and isobutrin (BI) was studied using cell culture of Normal Human Keratinocyte, cells involved in the skin inflammatory.MATERIALS AND METHODS:Dried and crushed B. monosperma flowers were extracted with Ethanol/H2O (70/30 v/v). The butrin/isobutrin fraction was obtained by centrifugal Partition Chromatography (CPC). Experiments were conducted on UV-B treated normal human epidermis keratinocytes, cells involved in the skin inflammatory response. To evaluate extract anti-inflammatory activity, cytokines IL-1β, IL-6, IL-8, prostaglandin E2 and metalloproteinases MMP-1, -2, -9 and -10 were measured in the cells supernatant.RESULTS:Our data clearly showed that hydroalcoholic B. monosperma flower extract was able to decrease the secretion of IL-1β, IL-6 and IL-8 pro-inflammatory cytokines of -32, -33 and -18% respectively. Interestingly, Prostaglandin E2 production and the secretion of MMP-1, -2, -9 and -10 were also inhibited. Same results were observed in presence of enriched fraction in butrin and isobutrin and confirmed the participation of these molecules in the anti-inflammatory activity.CONCLUSION:These results explain the anti-inflammatory activity of B. monosperma and confirm the interest to use it in traditional Indian medicine. Moreover, its metalloproteinases inhibitory activities coupled with its anti-inflammatory action also give anti-aging property to this plant. |
Databáze: | OpenAIRE |
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