Effects of fusion-activation interval and embryo aggregation on in vitro and in vivo development of bovine cloned embryos
| Autor: | Marcelo Bertolini, Eduardo S. Ribeiro, L. H. Aguiar, Felipe Ledur Ongaratto, Luciana Relly Bertolini, Maria Angélica Miglino, Fabiano Carminatti Zago, José Luiz Rodrigues, Fabiana Forell, Paula Rodriguez-Villamil, Carlos Eduardo Ambrósio, R. P. C. Gerger |
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| Rok vydání: | 2019 |
| Předmět: |
Nuclear Transfer Techniques
040301 veterinary sciences Somatic cell Cloning Organism Embryonic Development Biology Cleavage (embryo) Embryo Culture Techniques 0403 veterinary science Andrology 03 medical and health sciences Pregnancy In vivo medicine Animals Blastocyst CLONAGEM ANIMAL 030304 developmental biology Cloning 0303 health sciences Zygote General Veterinary Embryo 04 agricultural and veterinary sciences Embryo Mammalian In vitro medicine.anatomical_structure Oocytes Cattle Female |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Nuclear reprogramming in somatic cell cloning is one of the key factors for proper development, with variations in the protocol appearing to improve cloning efficiency. This study aimed to determine the effects of two fusion-activation intervals and the aggregation of bovine cloned embryos on subsequent in vitro and in vivo development. Zygotes produced by handmade cloning were exposed to two fusion-activation intervals (2 h or 4 h), and then cultured in microwells either individually (1 × 100%) or after aggregation of two structures (2 × 100%). Zona-intact oocytes and zona-free oocytes and hemi-oocytes were used as parthenote controls under the same fusion-activation intervals. Day-7 cloned blastocysts were transferred to synchronous recipients. Cleavage (Day 2), blastocyst (Day 7) and pregnancy (Day 30) rates were compared by the χ2 test (P |
| Databáze: | OpenAIRE |
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