A molecular 'signature' of primary breast cancer cultures; patterns resembling tumor tissue
| Autor: | Yong Ben, Stefanie S. Jeffrey, Zhenhang Meng, Dan H. Moore, Shanaz H. Dairkee, Youngran Ji |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Telomerase
lcsh:QH426-470 lcsh:Biotechnology Down-Regulation Breast Neoplasms Biology lcsh:TP248.13-248.65 Cell Line Tumor Gene expression Genetics medicine Tumor Cells Cultured Humans Telomerase reverse transcriptase Breast Cell Proliferation Oligonucleotide Array Sequence Analysis CENPA Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Epithelial Cells medicine.disease Primary tumor Molecular biology Up-Regulation Gene expression profiling lcsh:Genetics Cell culture Multigene Family Cancer research CDK inhibitor Biotechnology Research Article |
| Zdroj: | BMC Genomics BMC Genomics, Vol 5, Iss 1, p 47 (2004) |
| ISSN: | 1471-2164 |
| Popis: | Background To identify the spectrum of malignant attributes maintained outside the host environment, we have compared global gene expression in primary breast tumors and matched short-term epithelial cultures. Results In contrast to immortal cell lines, a characteristic 'limited proliferation' phenotype was observed, which included over expressed genes associated with the TGFβ signal transduction pathway, such as SPARC, LOXL1, RUNX1, and DAPK1. Underlying this profile was the conspicuous absence of hTERT expression and telomerase activity, a significant increase in TβRII, its cognate ligand, and the CDK inhibitor, p21CIP1/WAF1. Concurrently, tumor tissue and primary cultures displayed low transcript levels of proliferation-related genes, such as, TOP2A, ANKT, RAD51, UBE2C, CENPA, RRM2, and PLK. Conclusions Our data demonstrate that commonly used immortal cell lines do not reflect some aspects of tumor biology as closely as primary tumor cell cultures. The gene expression profile of malignant tissue, which is uniquely retained by cells cultured on solid substrates, could facilitate the development and testing of novel molecular targets for breast cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink