Effective Utilization of Carbohydrate in Corncob to Synthesize Furfuralcohol by Chemical-Enzymatic Catalysis in Toluene-Water Media
| Autor: | Bing-Qian Wang, Yu-Cai He, Cui-Luan Ma, Jun-Hua Di, Xin-Xia Xue |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Arabinose Oxalic acid Bioengineering Xylose Corncob Furfural 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Zea mays chemistry.chemical_compound Hydrolysis Polysaccharides 010608 biotechnology Escherichia coli Hemicellulose Cellulose Furans Molecular Biology 010405 organic chemistry Escherichia coli Proteins Water General Medicine 0104 chemical sciences chemistry Oxidoreductases Biotechnology Nuclear chemistry Toluene |
| Zdroj: | Applied biochemistry and biotechnology. 185(1) |
| ISSN: | 1559-0291 |
| Popis: | In this study, carbohydrates (cellulose plus hemicellulose) in corncob were effectively converted furfuralcohol (FOL) via chemical-enzymatic catalysis in a one-pot manner. After corncob (2.5 g, dry weight) was pretreated with 0.5 wt% oxalic acid, the obtained corncob-derived xylose (19.8 g/L xylose) could be converted to furfural at 60.1% yield with solid acid catalyst SO42-/SnO2-attapulgite (3.6 wt% catalyst loading) in the water-toluene (3:1, v/v) at 170 °C for 20 min. Moreover, the oxalic acid-pretreated corncob residue (1.152 g, dry weight) was enzymatically hydrolyzed to 0.902 g glucose and 0.202 g arabinose. Using the corncob-derived glucose (1.0 mM glucose/mM furfural) as cosubstrate, the furfural liquor (48.3 mM furfural) was successfully biotransformed to FOL by recombinant Escherichia coli CCZU-A13 cells harboring an NADH-dependent reductase (SsCR) in the water-toluene (4:1, v/v) under the optimum conditions (50 mM PEG-6000, 0.2 mM Zn2+, 0.1 g wet cells/mL, 30 °C, pH 6.5). After the bioreduction for 2 h, FAL was completely converted to FOL. The FOL yield was obtained at 0.11 g FOL/g corncob. Clearly, this one-pot synthesis strategy shows high potential application for the effective synthesis of FOL. |
| Databáze: | OpenAIRE |
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