A rapid extraction technique for atomic absorption determinations of kidney calcium
| Autor: | William P. Tew, Charles D. Malis, W. Gordon Walker |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
inorganic chemicals
Male Hot Temperature Sonication Butanols Biophysics Analytical chemistry chemistry.chemical_element Calcium Kidney Biochemistry law.invention Mice law Lanthanum medicine Animals Molecular Biology Chromatography Chemistry Spectrophotometry Atomic Extraction (chemistry) Cell Biology medicine.disease medicine.anatomical_structure Ashing Hydrochloric Acid Nephrocalcinosis Atomic absorption spectroscopy Calcification |
| Zdroj: | Analytical biochemistry. 112(2) |
| ISSN: | 0003-2697 |
| Popis: | A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily. |
| Databáze: | OpenAIRE |
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