Gene expression signature of chronic lymphocytic leukaemia with Trisomy 12
| Autor: | Berthold Streubel, E. Porpaczy, Michaela Gruber, Ilse Schwarzinger, Christa Fonatsch, Ulrich Jaeger, Katrina Vanura, Georg Heinze, Martin Bilban, Stephan Stilgenbauer |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Chronic lymphocytic leukemia Clinical Biochemistry Trisomy Biology Biochemistry Gene expression medicine Humans Chromosome 12 Oligonucleotide Array Sequence Analysis Chromosomes Human Pair 12 Gene Expression Regulation Leukemic Gene Expression Profiling General Medicine Middle Aged medicine.disease Prognosis Molecular biology Leukemia Lymphocytic Chronic B-Cell Gene expression profiling Leukemia Real-time polymerase chain reaction Chromosome 3 Karyotyping Immunology Female |
| Zdroj: | European journal of clinical investigation. 39(7) |
| ISSN: | 1365-2362 |
| Popis: | Background The prognosis of chronic lymphocytic leukaemia (CLL) patients is largely determined by the karyotype of the malignant clone. We have investigated the gene expression profile associated with trisomy 12 (+12). Design Initially, unselected peripheral blood mononuclear cells of four patients with +12 were compared with 16 CLL controls using microarray analysis. Results were validated by quantitative real-time PCR with RNA from 61 patients (29 with +12, 32 CLL controls). Results Seven genes showing the strongest correlation with +12 in microarray analysis were selected for real-time PCR: HIP1R, MYF6, SLC2A6, CD9 (overexpressed); CD200, P2RY14, RASGRP3 (underexpressed). Four genes were significantly associated with +12: HIP1R (P |
| Databáze: | OpenAIRE |
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