Broccoli microgreens juice reduces body weight by enhancing insulin sensitivity and modulating gut microbiota in high-fat diet-induced C57BL/6J obese mice
| Autor: | Yunfan Wang, Xiangfei Li, Yingjian Lu, Shuhua Tian, Jing Wang, Jie Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Firmicutes medicine.medical_treatment Mice Obese Medicine (miscellaneous) Adipose tissue 030209 endocrinology & metabolism Brassica Biology Gut flora Diet High-Fat medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Adipocyte Internal medicine medicine Animals Obesity health care economics and organizations 030109 nutrition & dietetics Nutrition and Dietetics Insulin medicine.disease biology.organism_classification Gastrointestinal Microbiome Mice Inbred C57BL Endocrinology chemistry Insulin Resistance Oxidative stress |
| Zdroj: | European Journal of Nutrition. 60:3829-3839 |
| ISSN: | 1436-6215 1436-6207 |
| Popis: | This study aimed to explore the protective effect of broccoli microgreens juice (BMJ) during C57BL/6J mice obesity development. The obese model mice, induced by feeding high-fat diet (HFD), were treated with BMJ by gavage for 10 weeks. Melbine was gavaged at 300 mg/(kg bw)/d, as a positive control group. BMJ supplementation significantly reduced white adipose tissues (WAT) mass, the body weight and adipocyte size, and increased water intake in HFD-fed mice. Moreover, it improved glucose tolerance, reduced insulin level and HOMA-IR value, and alleviated insulin resistance. Compared with the HFD group, BMJ supplementation significantly increased the relative abundance of Bacteroidetes and decreased the ratio of Firmicutes to Bacteroidetes at the phylum level, and enriched Bacteroides_acidifaciens at the species level. These changes in the composition of gut microbiota are associated with the production of short-chain fatty acids (SCFAs), and reduced LPS levels, and had an obvious anti-inflammatory effect. These findings suggested that the protective effects of BMJ on diet-induced obesity may be involved in gut microbiota–SCFAs–LPS–inflammatory axis. In addition, BMJ can enhance liver antioxidant capacity and reduce liver fat accumulation. Consequently, these results sustain BMJ as a novel functional food for obesity, on the basis of its opposing effects on HFD-induced obesity in mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|