The Modulation of Cholesterol Metabolism Is Involved in the Antiviral Effect of Nitazoxanide
| Autor: | Sergio Strizzi, Mario Clerici, Claudia Vanetti, Claudio Fenizia, Mara Biasin, Salomè Valentina Ibba, Daria Trabattoni, Jean Francois Rossignol |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
FLU 030106 microbiology Cell Pharmacology Article 03 medical and health sciences Other systems of medicine Immune system parasitic diseases nitazoxanide Medicine Innate immune system business.industry Lipid metabolism Nitazoxanide interferon thiazolide antiviral In vitro 030104 developmental biology Infectious Diseases medicine.anatomical_structure Viral replication cholesterol metabolism lipids (amino acids peptides and proteins) Efflux business RZ201-999 medicine.drug |
| Zdroj: | Infectious Disease Reports, Vol 13, Iss 60, Pp 636-644 (2021) Infectious Disease Reports Volume 13 Issue 3 Pages 60-644 |
| ISSN: | 2036-7449 |
| Popis: | We previously investigated the role of Nitazoxanide (NTZ), a thiazolide endowed with antiviral and antiparasitic activity, in HIV-1 infection. NTZ treatment in primary isolated PBMCs was able to reduce HIV-1 infection in vitro by inducing the expression of a number of type-I interferon-stimulated genes. Among them, NTZ was able to induce cholesterol-25-hydroxylase (CH25H), which is involved in cholesterol metabolism. In the present study, we wanted to deepen our knowledge about the antiviral mechanism of action of NTZ. Indeed, by inducing CH25H, which catalyzes the formation of 25-hydroxycholesterol from cholesterol, NTZ treatment repressed cholesterol biosynthetic pathways and promoted cholesterol mobilization and efflux from the cell. Such effects were even more pronounced upon stimulation with FLU antigens in combination. It is already well known how lipid metabolism and virus replication are tightly interconnected thus, it is not surprising that the antiviral immune response employs genes related to cholesterol metabolism. Indeed, NTZ was able to modulate cholesterol metabolism in vitro and, by doing so, enhance the antiviral response. These results give us the chance to speculate about the suitability of NTZ as adjuvant for induction of specific natural immunity. Moreover, the putative application of NTZ to alimentary-related diseases should be investigated. |
| Databáze: | OpenAIRE |
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