A Potential miRNA-mRNA Network for Dementia and Hernia Crosstalk
| Autor: | De-jian Chen, Da-peng Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Candidate gene Hernia Article Subject Gene regulatory network Down-Regulation Adipose tissue Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Gene expression microRNA medicine Humans Dementia Gene Regulatory Networks RNA Messenger General Immunology and Microbiology Gene Expression Profiling General Medicine medicine.disease Neoplasm Proteins Up-Regulation Gene expression profiling MicroRNAs Gene Ontology HEK293 Cells 030104 developmental biology Cancer research Medicine Biomarker (medicine) 030217 neurology & neurosurgery Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Background. It has been reported that there may be a potential link between hernia and dementia. However, the exact mechanisms of their association have not been established. This study is aimed at constructing miRNA-mRNA networks to elucidate on the potential link between dementia and hernia. Methods. Gene expression profiles for dementia, herniation, and skeletal muscle were downloaded from the GEO database after which differentially expressed mRNAs and miRNAs were obtained. In addition, fascia tissue samples were obtained during surgery. A total of 41 patients were recruited in this study, and expression levels of candidate genes were examined using quantitative RT-PCR. Luciferase reporter gene assays were used to identify potential miRNA-mRNA regulatory pathways. Results. Differentially expressed mRNAs and miRNAs were screened. A potential miRNA-mRNA network revealing the crosstalk mechanism between herniation and dementia was identified. Single cell analysis revealed that PI16 was highly enriched in adipose tissues, skeletal muscles, and in the skin. GSEA enrichment analysis showed that PI16 is involved in adipose metabolism, muscle functions, and energy metabolism. In clinical samples, PI16 was found to be upregulated in hernia, while miR-4451 was found to be downregulated. The luciferase reporter gene assay revealed that downregulation of circulating miR-4451 may be responsible for the upregulated PI16 expression in hernia sacs. Conclusions. We constructed an miRNA-mRNA network that shows the potential association between dementia and hernia. We also found that miR-4451 regulates the PI16 expression, which may be a key target and biomarker for hernia pathogenesis and dementia crosstalk. |
| Databáze: | OpenAIRE |
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