Niche-adaptation in plant-associated Bacteroidetes favours specialisation in organic phosphorus mineralisation
| Autor: | Gary D. Bending, Chiara Borsetto, Alexandra M. E. Jones, Yin Chen, David J. Scanlan, Andrew R. J. Murphy, Ian Lidbury, John P. Hammond, Elizabeth M. H. Wellington, Andrew R. Bottrill |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Rhizosphere biology 030306 microbiology Phosphorus Niche Phosphatase chemistry.chemical_element Bacteroidetes Genomic signature biology.organism_classification Microbiology 03 medical and health sciences chemistry Evolutionary biology QR180 Microbiome Niche adaptation Ecology Evolution Behavior and Systematics 030304 developmental biology |
| Zdroj: | The ISME Journal. 15:1040-1055 |
| ISSN: | 1751-7370 1751-7362 |
| Popis: | Bacteroidetes are abundant pathogen-suppressing members of the plant microbiome that contribute prominently to rhizosphere phosphorus mobilisation, a frequent growth-limiting nutrient in this niche. However, the genetic traits underpinning their success in this niche remain largely unknown, particularly regarding their phosphorus acquisition strategies. By combining cultivation, multi-layered omics and biochemical analyses we first discovered that all plant-associated Bacteroidetes express constitutive phosphatase activity, linked to the ubiquitous possession of a unique phosphatase, PafA. For the first time, we also reveal a subset of Bacteroidetes outer membrane SusCD-like complexes, typically associated with carbon acquisition, and several TonB-dependent transporters, are induced during Pi-depletion. Furthermore, in response to phosphate depletion, the plant-associated Flavobacterium used in this study expressed many previously characterised and novel proteins targeting organic phosphorus. Collectively, these enzymes exhibited superior phosphatase activity compared to plant-associated Pseudomonas spp. Importantly, several of the novel low-Pi-inducible phosphatases and transporters, belong to the Bacteroidetes auxiliary genome and are an adaptive genomic signature of plant-associated strains. In conclusion, niche adaptation to the plant microbiome thus appears to have resulted in the acquisition of unique phosphorus scavenging loci in Bacteroidetes, enhancing their phosphorus acquisition capabilities. These traits may enable their success in the rhizosphere and also present exciting avenues to develop sustainable agriculture. |
| Databáze: | OpenAIRE |
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