Co-Detection of miR-21 and TNF-α mRNA in Budding Cancer Cells in Colorectal Cancer

Autor: Jaslin P James, Flemming Brandt Sørensen, Kim Holmstrøm, Trine Møller, Jan Lindebjerg, Boye Schnack Nielsen
Rok vydání: 2019
Předmět:
Stromal cell
Colon
Colorectal cancer
medicine.medical_treatment
colorectal cancer
Confocal slide scanning microscopy
Microrna
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
Paracrine signalling
tumor budding cells
microRNA
Humans
Medicine
RNA
Messenger
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Inflammation
Tumor budding cells
Microscopy
Confocal
Tumor Necrosis Factor-alpha
confocal slide scanning microscopy
business.industry
interleukin-1β
microRNA
Organic Chemistry
Cancer
General Medicine
medicine.disease
Interleukin-1β
Computer Science Applications
Gene Expression Regulation
Neoplastic
MicroRNAs
Cytokine
lcsh:Biology (General)
lcsh:QD1-999
inflammation
MiR-21
TNF-α
Cancer cell
Cancer research
Tumor necrosis factor alpha
miR-21
Colorectal Neoplasms
business
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 8
International Journal of Molecular Sciences, Vol 20, Iss 8, p 1907 (2019)
Møller, T, James, J P, Holmstrøm, K, Sørensen, F B, Lindebjerg, J & Nielsen, B S 2019, ' Co-detection of miR-21 and TNF-α mRNA in budding cancer cells in colorectal cancer ', International Journal of Molecular Sciences, vol. 20, no. 8, 1907 . https://doi.org/10.3390/ijms20081907
ISSN: 1422-0067
Popis: MicroRNA-21 (miR-21) is upregulated in many cancers including colon cancers and is a prognostic indicator of recurrence and poor prognosis. In colon cancers, miR-21 is highly expressed in stromal fibroblastic cells and more weakly in a subset of cancer cells, particularly budding cancer cells. Exploration of the expression of inflammatory markers in colon cancers revealed tumor necrosis factor alpha (TNF-&alpha
) mRNA expression at the invasive front of colon cancers. Surprisingly, a majority of the TNF-&alpha
mRNA expressing cells were found to be cancer cells and not inflammatory cells. Because miR-21 is positively involved in cell survival and TNF-&alpha
promotes necrosis, we found it interesting to analyze the presence of miR-21 in areas of TNF-&alpha
mRNA expression at the invasive front of colon cancers. For this purpose, we developed an automated procedure for the co-staining of miR-21, TNF-&alpha
mRNA and the cancer cell marker cytokeratin based on analysis of frozen colon cancer tissue samples (n = 4) with evident cancer cell budding. In all four cases, TNF-&alpha
mRNA was seen in a small subset of cancer cells at the invasive front. Evaluation of miR-21 and TNF-&alpha
mRNA expression was performed on digital slides obtained by confocal slide scanning microscopy. Both co-expression and lack of co-expression with miR-21 in the budding cancer cells was noted, suggesting non-correlated expression. miR-21 was more often seen in cancer cells than TNF-&alpha
mRNA. In conclusion, we report that miR-21 is not linked to expression of the pro-inflammatory cytokine TNF-&alpha
mRNA, but that miR-21 and TNF-&alpha
both take part in the cancer expansion at the invasive front of colon cancers. We hypothesize that miR-21 may protect both fibroblasts and cancer cells from cell death directed by TNF-&alpha
paracrine and autocrine activity.
Databáze: OpenAIRE
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