a multifunctional pipette
| Autor: | Aldo Jesorka, Alar Ainla, Owe Orwar, Ralf Brune, Gavin D. M. Jeffries |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Engineering
Sample processing Microfluidics Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology CHO Cells 01 natural sciences Biochemistry Article Specimen Handling Cricetulus Cricetinae Microscopy Fluorescence microscope Animals Sample preparation Total internal reflection fluorescence microscope business.industry 010401 analytical chemistry Pipette General Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Solutions 0210 nano-technology business |
| Zdroj: | Lab on a Chip |
| ISSN: | 1473-0197 |
| DOI: | 10.1039/c2lc20906c |
| Popis: | Microfluidics has emerged as a powerful laboratory toolbox for biologists, allowing manipulation and analysis of processes at a cellular and sub-cellular level, through utilization of microfabricated features at size-scales relevant to that of a single cell. In the majority of microfluidic devices, sample processing and analysis occur within closed microchannels, imposing restrictions on sample preparation and use. We present an optimized non-contact open-volume microfluidic tool to overcome these and other restrictions, through the use of a hydrodynamically confined microflow pipette, serving as a multifunctional solution handling and dispensing tool. The geometries of the tool have been optimised for use in optical microscopy, with integrated solution reservoirs to reduce reagent use, contamination risks and cleaning requirements. Device performance was characterised using both epifluorescence and total internal reflection fluorescence (TIRF) microscopy, resulting in ∼200 ms and ∼130 ms exchange times at ∼100 nm and ∼30 μm distances to the surface respectively. |
| Databáze: | OpenAIRE |
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