Osteogenic stimulation of human dental pulp stem cells with self‐setting biphasic calcium phosphate cement
| Autor: | Yingzhi Gu, Xianju Xie, Rui Zhuang, Yuxing Bai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biocompatibility Biomedical Engineering Macrophage polarization macromolecular substances 02 engineering and technology Proinflammatory cytokine Biomaterials 03 medical and health sciences chemistry.chemical_compound stomatognathic system Osteogenesis Dental pulp stem cells Materials Testing Humans Bone regeneration Dental Pulp 030304 developmental biology 0303 health sciences Stem Cells Regeneration (biology) Bone Cements technology industry and agriculture Tetracalcium phosphate ttcp 021001 nanoscience & nanotechnology Cell biology chemistry Hydroxyapatites 0210 nano-technology |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 108:1669-1678 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.34512 |
| Popis: | Calcium phosphate cement (CPC) is widely used as a repair material for bone defects. However, there is no report on the use of biphasic CPC combined with dental pulp stem cells (DPSCs) for bone tissue engineering. The aim of this study was to construct biphasic CPC and to investigate its effect in the osteogenic stimulation of human DPSCs (hDPSCs) and macrophage polarization. Biphasic CPC was developed by adding different ratios of β-TCP to an equimolar mixture of tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA). The biocompatibility, osteogenic capacity, and inflammatory reactions of the cements were examined. Our results show that biphasic CPC containing both HA and β-TCP was successfully fabricated. The addition of β-TCP did not increase necrosis or apoptosis of DPSCs. Significant increases in hDPSC mineral deposition and osteogenic marker (ColI and ALP) expression were observed in 20% TCP-CPC. The addition of β-TCP did not promote macrophage polarization to a proinflammatory phenotype. In conclusion, biphasic CPC with hDPSCs for bone regeneration was developed for the first time; the biphasic CPC combined with hDPSCs is expected to be used for the repair and regeneration of bone. |
| Databáze: | OpenAIRE |
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