Structurally distinct external solvent-exposed domains drive replication of major human prions
| Autor: | Mark R. Chance, Miroslava Kacirova, Jiri G. Safar, Mohammad Khursheed Siddiqi, Chae Kim, Janna Kiselar, Jen Bohon, Tracy Haldiman, Benlian Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chemical Radicals
PrPSc Proteins Protein Conformation animal diseases Biochemistry Physical Chemistry Creutzfeldt-Jakob Syndrome Animal Diseases 0302 clinical medicine Protein structure Prion infectivity Zoonoses Protein Isoforms Hydroxyl Radicals Post-Translational Modification Biology (General) A determinant 0303 health sciences Structural organization Chemistry Neurodegenerative diseases Phenotype Recombinant Proteins Cell biology Animal Prion Diseases Neurology Propagation rate Physical Sciences Infectious diseases Engineering and Technology Research Article Medical conditions Prion diseases Prions QH301-705.5 Immunology Protein domain Equipment DNA footprinting Hydroxylation Microbiology 03 medical and health sciences Protein Domains Virology Genetics Humans Molecular Biology 030304 developmental biology Medicine and health sciences Biology and Life Sciences Proteins RC581-607 Creutzfeldt-Jakob disease nervous system diseases Parasitology Particle Accelerators Immunologic diseases. Allergy Zoology Synchrotrons 030217 neurology & neurosurgery |
| Zdroj: | PLoS Pathogens, Vol 17, Iss 6, p e1009642 (2021) PLoS Pathogens |
| ISSN: | 1553-7374 1553-7366 |
| Popis: | There is a limited understanding of structural attributes that encode the iatrogenic transmissibility and various phenotypes of prions causing the most common human prion disease, sporadic Creutzfeldt-Jakob disease (sCJD). Here we report the detailed structural differences between major sCJD MM1, MM2, and VV2 prions determined with two complementary synchrotron hydroxyl radical footprinting techniques—mass spectrometry (MS) and conformation dependent immunoassay (CDI) with a panel of Europium-labeled antibodies. Both approaches clearly demonstrate that the phenotypically distant prions differ in a major way with regard to their structural organization, and synchrotron-generated hydroxyl radicals progressively inhibit their seeding potency in a strain and structure-specific manner. Moreover, the seeding rate of sCJD prions is primarily determined by strain-specific structural organization of solvent-exposed external domains of human prion particles that control the seeding activity. Structural characteristics of human prion strains suggest that subtle changes in the organization of surface domains play a critical role as a determinant of human prion infectivity, propagation rate, and targeting of specific brain structures. Author summary Sporadic human prion diseases are conceivably the most heterogenous neurodegenerative disorders and a growing body of research indicates that they are caused by distinct strains of prions. By parallel monitoring their replication potency and progressive hydroxyl radical modification of amino acid side chains during synchrotron irradiation, we identified major differences in the structural organization that correlate with distinct inactivation susceptibility of a given human prion strain. Furthermore, our data demonstrated, for the first time, that seeding activity of different strains of infectious brain-derived human prions is primarily function of distinct solvent-exposed structural domains, and implicate them in the initial binding of cellular isoform of prion protein (PrPC) as a critical step in human prion replication and infectivity. |
| Databáze: | OpenAIRE |
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