Optimization of the Sulfo-Phospho-Vanillin Assay for Total Lipid Normalization in Untargeted Quantitative Lipidomic LC–MS/MS Applications

Autor:	Laura S. Bailey, Dilip V. Prajapati, Kari B. Basso
Rok vydání:	2022
Předmět:	Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:17810-17818
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.2c03488
Popis:	Liquid chromatography (LC)-mass spectrometry (MS)/MS lipidomic normalization is generally performed by equalizing pre-extraction sample materials or via DNA or protein pre-quantitation methods, which have known measurement inaccuracies. We propose the use of the sulfo-phospho-vanillin assay (SPVA), a total lipid colorimetric analysis, as a pre-quantitation method to normalize lipids in lipidomic LC-MS/MS applications. The assay has been applied to a 300 μL well volume in a 96-well plate and tested using Avanti total lipid standards of porcine brain and
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00fe0cf415f28daa62a5e4ab2b0475b9 https://doi.org/10.1021/acs.analchem.2c03488 Zobrazit plný text záznamu